pCJ203
(Plasmid #162678)

• Purpose: pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
• Depositing Lab: Gregg Beckham
• Publication: Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;117(41):25476-25485. doi: 10.1073/pnas.2006753117. Epub 2020 Sep 28.

Backbone

• Vector backbone: pET-21b(+)
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5367
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
• Species: Comamonas thiooxydans
• Mutation: deltaF2-A75; codon optimized for expression in E. coli K12
• Promoter: T7/lac
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pCJ203.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with a deletion of the predicted signal peptide (Phe2:Ala75), with C-terminal His tag, codon optimized for expression in E. coli K12.