METTL3 shRNA 2 tet pLKO puro
(Plasmid #162984)

• Purpose: Tet-inducible shRNA targeting human METTL3 #2
• Depositing Lab: Mark Rubin
• Publication: Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-7786.MCR-21-0014. doi: 10.1158/1541-7786.MCR-21-0014.

Backbone

• Vector backbone: Tet-pLKO-puro
• Backbone size w/o insert (bp): 8762
• Total vector size (bp): 8816
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: methyltransferase like 3
• Alt name: METTL3
• gRNA/shRNA sequence: GCTGCACTTCAGACGAATTAT
• Species: H. sapiens (human)
• GenBank ID: NM_019852
• Entrez Gene: METTL3 (a.k.a. IME4, M6A, MT-A70, Spo8, hMETTL3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GGCAGGGATATTCACCATTATCGTTTCAGA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original backbone from Dmitri Wiederschain (Addgene plasmid # 21915)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry