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pLyGo-Ec-7
(Plasmid #163135)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 163135 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD
  • Backbone size (bp) 6663
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter P(rhaB)
  • Tag / Fusion Protein
    • Nanobody, C-IgAP autotransporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLyGo-Ec-7 was a gift from Morten Norholm (Addgene plasmid # 163135 ; http://n2t.net/addgene:163135 ; RRID:Addgene_163135)
  • For your References section:

    LyGo: A Platform for Rapid Screening of Lytic Polysaccharide Monooxygenase Production. Hernandez-Rollan C, Falkenberg KB, Rennig M, Bertelsen AB, Ipsen JO, Brander S, Daley DO, Johansen KS, Norholm MHH. ACS Synth Biol. 2021 Apr 16;10(4):897-906. doi: 10.1021/acssynbio.1c00034. Epub 2021 Apr 2. 10.1021/acssynbio.1c00034 PubMed 33797234