|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16336||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneNr6a1 (a.k.a. 1700113M01Rik, Gcnf, NCNF, RTR)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
Compared to GenBank accession number NP_034394.1, the GCNF insert in this plasmid contains N33S and N414D mutations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-HA GCNF was a gift from Austin Cooney (Addgene plasmid # 16336 ; http://n2t.net/addgene:16336 ; RRID:Addgene_16336)
For your References section:Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Gu P, LeMenuet D, Chung AC, Mancini M, Wheeler DA, Cooney AJ. Mol Cell Biol. 2005 Oct . 25(19):8507-19. 10.1128/MCB.25.19.8507-8519.2005 PubMed 16166633