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(Plasmid #163674)


Item Catalog # Description Quantity Price (USD)
Plasmid 163674 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    E. Snapp (Addgene plasmid # 68070)
  • Backbone size w/o insert (bp) 4695
  • Total vector size (bp) 5040
  • Modifications to backbone
    The original NdeI in pmoxGFP-N1 is abolished.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    For this partial length ST chimera, two restriction sites, SpeI and NdeI, were introduced into the TMD by silent muation and its TMD length is elongated to 19 amino acids by inserting Ala and Val residues.
  • GenBank ID
  • Entrez Gene
    ST6GAL1 (a.k.a. SIAT1, ST6GalI, ST6N)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMD19 was a gift from Lei Lu (Addgene plasmid # 163674 ; ; RRID:Addgene_163674)
  • For your References section:

    A quantitative study of the Golgi retention of glycosyltransferases. Sun X, Mahajan D, Chen B, Song Z, Lu L. J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21. 10.1242/jcs.258564 PubMed 34533190