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A quantitative study of the Golgi retention of glycosyltransferases.
Sun X, Mahajan D, Chen B, Song Z, Lu L
J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
163645Man1B1-MycExpresses myc tagged Man1B1 in mammalian cells
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163646Man1B1-GFPExpresses GFP tagged Man1B1 in mammalian cells
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163647MGAT1-GFPExpresses GFP tagged MGAT1 in mammalian cells
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163648Man2a1-mycExpresses myc tagged Man2a1 in mammalian cells
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163649Man2a1-GFPExpresses GFP tagged Man2a1 in mammalian cells
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163654FSS-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cells
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163655SFS-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells
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163656SSF-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
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163657SFF-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cells
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163658FSF-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
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163659FFS-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cells
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163660TNFa-GFPExpresses GFP tagged TNFa in mammalian cells
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163661NSS-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells
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163662SNS-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells
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163663SSN-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells
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163664SNN-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells
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163665NSN-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF, TMD from ST6GAL1 and luminal domain from TNF in mammalian cells
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163666NNS-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells
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163667ST*-GFPExpresses partial length ST in mammalian cells
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163668SBP-ST*-GFPExpresses SBP tagged partial length ST in mammalian cells
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163669FKBP-ST*-GFPExpresses FKBP tagged partial length ST in mammalian cells
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163670F9SS-GFPExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, and TMD and luminal domain from ST6GAL1 in mammalian cells
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163671F9SF-GFPExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cells
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163672F9FS-GFPExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells
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163673TMD18Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
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163674TMD19Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
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163675TMD20Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells
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163676TMD21Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells
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163677TMD22Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells
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163678TMD24Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells
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163679F9F17F-GFPExpresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells
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163680ss-ED-mCherry-GPIExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
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163858VPS35 shRNA1shRNA targeting the coding sequences of VPS35
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163859VPS35 shRNA2shRNA targeting the coding sequences of VPS35
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163860GOLPH3 shRNAshRNA targeting the coding sequences of GOLPH3
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163861COG4 shRNAshRNA targeting the coding sequences of COG4
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Recombinant Antibodies from Article