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A quantitative study of the Golgi retention of glycosyltransferases.
Sun X, Mahajan D, Chen B, Song Z, Lu L
J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
163645Man1B1-MycExpresses myc tagged Man1B1 in mammalian cells
163646Man1B1-GFPExpresses GFP tagged Man1B1 in mammalian cells
163647MGAT1-GFPExpresses GFP tagged MGAT1 in mammalian cells
163648Man2a1-mycExpresses myc tagged Man2a1 in mammalian cells
163649Man2a1-GFPExpresses GFP tagged Man2a1 in mammalian cells
163654FSS-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cells
163655SFS-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells
163656SSF-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
163657SFF-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cells
163658FSF-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
163659FFS-GFPExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cells
163660TNFa-GFPExpresses GFP tagged TNFa in mammalian cells
163661NSS-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells
163662SNS-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells
163663SSN-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells
163664SNN-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells
163665NSN-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF, TMD from ST6GAL1 and luminal domain from TNF in mammalian cells
163666NNS-GFPExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells
163667ST*-GFPExpresses partial length ST in mammalian cells
163668SBP-ST*-GFPExpresses SBP tagged partial length ST in mammalian cells
163669FKBP-ST*-GFPExpresses FKBP tagged partial length ST in mammalian cells
163670F9SS-GFPExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, and TMD and luminal domain from ST6GAL1 in mammalian cells
163671F9SF-GFPExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cells
163672F9FS-GFPExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells
163673TMD18Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
163674TMD19Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
163675TMD20Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells
163676TMD21Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells
163677TMD22Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells
163678TMD24Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells
163679F9F17F-GFPExpresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells
163680ss-ED-mCherry-GPIExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
163858VPS35 shRNA1shRNA targeting the coding sequences of VPS35
163859VPS35 shRNA2shRNA targeting the coding sequences of VPS35
163860GOLPH3 shRNAshRNA targeting the coding sequences of GOLPH3
163861COG4 shRNAshRNA targeting the coding sequences of COG4

Antibodies from Article