ss-ED-mCherry-GPI
(Plasmid
#163680)
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PurposeExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
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Depositing Lab
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Depositing OrganizationNanyang Technological University
Located in Singapore -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163680 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 163680-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 163680-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmoxGFP-N1
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Backbone manufacturerE. Snapp (Addgene plasmid # 68070)
- Backbone size w/o insert (bp) 3958
- Total vector size (bp) 5965
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameThe luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences
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Alt nameST6GAL1
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Alt nameST
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SpeciesH. sapiens (human)
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Insert Size (bp)2007
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MutationThis chimera contains the ecto-domain of ST. Its N-terminus was fused with a signal peptide and C-terminus was tagged with mCherry and GPI anchor.
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GenBank IDBC040009
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Entrez GeneST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- GPI anchor (C terminal on insert)
- signal sequences (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-PArev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 163680-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 163680-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ss-ED-mCherry-GPI was a gift from Lei Lu (Addgene plasmid # 163680 ; http://n2t.net/addgene:163680 ; RRID:Addgene_163680) -
For your References section:
A quantitative study of the Golgi retention of glycosyltransferases. Sun X, Mahajan D, Chen B, Song Z, Lu L. J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21. 10.1242/jcs.258564 PubMed 34533190
