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Addgene

pBS-mPax3
(Plasmid #16427)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 16427 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    In Situ Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Pax3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    345
  • Mutation
    None
  • Entrez Gene
    Pax3 (a.k.a. Pax-3, Sp, Splchl2, splotch)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modification of pBH3.2 Pax3 plasmid from Martyn Goulding, Max Plank Institute (Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P.
Pax-3, a novel murine DNA binding protein expressed during early neurogenesis.
EMBO J. 1991 10(5):1135-47). Deleted internal SmaI fragment using SmaI in polylinker and SmaI in insert.

Antisense probes are generated by digesting with EcoRI or HindIII and transcribing with T7 RNA Polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-mPax3 was a gift from Jonathan Epstein (Addgene plasmid # 16427 ; http://n2t.net/addgene:16427 ; RRID:Addgene_16427)