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Addgene

DCGS-10
(Plasmid #24752)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24752 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBSK-
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2958
  • Vector type
    in situ probe template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    (sex determining region Y)-box 10
  • Alt name
    Sox10
  • Alt name
    SRY-box10
  • Alt name
    Dom
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1450
  • Mutation
    3' end of Sox10 cDNA for in situ probe. Transcribe with T3 for sense; transcribe with T7 for antisense.
  • GenBank ID
    BC002824
  • Entrez Gene
    Sox10 (a.k.a. Dom, Sox21, gt)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DCGS-10 was a gift from William Pavan (Addgene plasmid # 24752 ; http://n2t.net/addgene:24752 ; RRID:Addgene_24752)
  • For your References section:

    Sox10 mutation disrupts neural crest development in Dom Hirschsprung mouse model. Southard-Smith EM, Kos L, Pavan WJ. Nat Genet. 1998 Jan . 18(1):60-4. 10.1038/ng0198-60 PubMed 9425902