Nup54 no FG-mEGFP494(1)
PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||164270||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5970
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationdeleted the FG region of Nup54
- Promoter CMV
/ Fusion Protein
- mEGFP (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Nup54 no FG-mEGFP494(1) was a gift from Sanford Simon (Addgene plasmid # 164270 ; http://n2t.net/addgene:164270 ; RRID:Addgene_164270)