Conformation of the nuclear pore in living cells is modulated by transport state.
Pulupa J, Prior H, Johnson DS, Simon SM
Elife. 2020 Dec 21;9. pii: 60654. doi: 10.7554/eLife.60654. PubMed Article
Pulupa J, Prior H, Johnson DS, Simon SM
Elife. 2020 Dec 21;9. pii: 60654. doi: 10.7554/eLife.60654. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164269 | Nup54 no FG-mEGFP494(0) | Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP |
| 164270 | Nup54 no FG-mEGFP494(1) | Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP |
| 164271 | Nup54 no FG-mEGFP494(2) | Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP |