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GP821
(Plasmid #164552)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164552 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smoothened K434R
  • Species
    M. musculus (mouse)
  • Mutation
    One of the three Lysines in loop three of Smoothened changed to arginine (KKK to RKK). K434R
  • Entrez Gene
    Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
  • Promoter GgCryD1
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GP821 was a gift from Gregory Pazour (Addgene plasmid # 164552 ; http://n2t.net/addgene:164552 ; RRID:Addgene_164552)
  • For your References section:

    Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. Desai PB, Stuck MW, Lv B, Pazour GJ. J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 10.1083/jcb.201912104. 10.1083/jcb.201912104 PubMed 32435793