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Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.
Desai PB, Stuck MW, Lv B, Pazour GJ
J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 10.1083/jcb.201912104.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
164526MS03Expresses Sonic Hedgehog
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164527GP734Expresses Flag-Ub
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164528PD19Expresses Flag-Ub noK
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164529PD14Expresses HA-Ub
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164530PD13Expresses HA-Ub noK
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164531PD22Expresses Smo-FLAG
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164532GP736 Express Smo-FLAG-Ub
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164533GP777Expresses Smo noK-FLAG
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164534GP806Expresses Smo noK-FLAG-Ub
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164535GP807Expresses Smo noK-FLAG-Ub noK
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164536PD23Expresses SmoM2-FLAG
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164537GP735Expresses SmoM2-FLAG-Ub
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164538GP938Expresses SmoPi-FLAG
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164539GP940Expresses SmoPi-FLAG-Ub
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164540GP757Expresses Sstr3-FLAG
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164541GP758Expresses Sstr3-FLAG-Ub
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164542PD29Expresses Drd1-FLAG
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164543PD30Expresses Drd1-Ub
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164544GP799Expresses Smo-FLAG
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164545GP805Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
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164546GP802Expresses Smo-FLAG K348, 360R
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164547GP803Expresses Smo-FLAG K434, 444, 448R
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164548GP804Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
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164549GP824Expresses Smo-FLAG K434, 444R
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164550GP825Expresses Smo-FLAG K434, 448R
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164551GP826Expresses Smo-FLAG K444, 448R
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164552GP821Expresses Smo-FLAG K434R
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164553GP822Expresses Smo-FLAG K444R
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164554GP823Expresses Smo-FLAG K448R
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Recombinant Antibodies from Article