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Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.
Desai PB, Stuck MW, Lv B, Pazour GJ
J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 10.1083/jcb.201912104.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
164526MS03Expresses Sonic Hedgehog
164527GP734Expresses Flag-Ub
164528PD19Expresses Flag-Ub noK
164529PD14Expresses HA-Ub
164530PD13Expresses HA-Ub noK
164531PD22Expresses Smo-FLAG
164532GP736 Express Smo-FLAG-Ub
164533GP777Expresses Smo noK-FLAG
164534GP806Expresses Smo noK-FLAG-Ub
164535GP807Expresses Smo noK-FLAG-Ub noK
164536PD23Expresses SmoM2-FLAG
164537GP735Expresses SmoM2-FLAG-Ub
164538GP938Expresses SmoPi-FLAG
164539GP940Expresses SmoPi-FLAG-Ub
164540GP757Expresses Sstr3-FLAG
164541GP758Expresses Sstr3-FLAG-Ub
164542PD29Expresses Drd1-FLAG
164543PD30Expresses Drd1-Ub
164544GP799Expresses Smo-FLAG
164545GP805Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
164546GP802Expresses Smo-FLAG K348, 360R
164547GP803Expresses Smo-FLAG K434, 444, 448R
164548GP804Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
164549GP824Expresses Smo-FLAG K434, 444R
164550GP825Expresses Smo-FLAG K434, 448R
164551GP826Expresses Smo-FLAG K444, 448R
164552GP821Expresses Smo-FLAG K434R
164553GP822Expresses Smo-FLAG K444R
164554GP823Expresses Smo-FLAG K448R

Antibodies from Article