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Addgene

pDownstream1Lox
(Plasmid #164574)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164574 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR)
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Entrez Gene
    DHFR (a.k.a. DHFR1, DHFRP1, DYR)
  • Promoter PyHSP70 promoter (PY17X_0712100)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU)
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    FCY1 (a.k.a. YPR062W)
  • Entrez Gene
    FUR1 (a.k.a. YHR128W)
  • Promoter Bidirectional PbEF1alpha promoter (PBANKA_1133300)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDownstream1Lox was a gift from Olivier Silvie (Addgene plasmid # 164574 ; http://n2t.net/addgene:164574 ; RRID:Addgene_164574)