Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #164580)


Item Catalog # Description Quantity Price (USD)
Plasmid 164580 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3965
  • Total vector size (bp) 5297
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
    Mb Tet3.0RS (R2-3)
  • Alt name
  • Species
    Methanosarcina barkeri (engineered)
  • Insert Size (bp)
  • Mutation
    L170G, N311G, C313A
  • Promoter TacI

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCTCTCCCTTATGCGACTCCTG
  • 3′ sequencing primer CTTCTGCGTTCTGATTTAATCTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Mm tRNA[TAA]
  • Alt name
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
  • Promoter ProK

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCGGCCTGCTGACTTTCTCG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Machinery plasmid for incorporation of Tet3.0 at TAA codons.

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltraI-Tet3.0[TAA] was a gift from Ryan Mehl (Addgene plasmid # 164580 ; ; RRID:Addgene_164580)
  • For your References section:

    Genetic Incorporation of Two Mutually Orthogonal Bioorthogonal Amino Acids That Enable Efficient Protein Dual-Labeling in Cells. Bednar RM, Jana S, Kuppa S, Franklin R, Beckman J, Antony E, Cooley RB, Mehl RA. ACS Chem Biol. 2021 Nov 19;16(11):2612-2622. doi: 10.1021/acschembio.1c00649. Epub 2021 Sep 30. 10.1021/acschembio.1c00649 PubMed 34590824