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GLuc-PM-Venus
(Plasmid #164785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164785 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 164785-D50 50 μg of DNA in Tris buffer $495
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DNA 164785-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3959
  • Total vector size (bp) 5507
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus
  • Alt name
    GLuc
  • Alt name
    PDGFRB
  • Alt name
    YFP
  • Species
    H. sapiens (human); Gaussia princeps
  • Insert Size (bp)
    1548
  • Entrez Gene
    PDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, OPDKD, PDGFR, PDGFR-1, PDGFR1, PENTT)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GLuc (N terminal on insert)
    • mVenus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII, XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GLuc-PM-Venus was a gift from Laszlo Hunyady (Addgene plasmid # 164785 ; http://n2t.net/addgene:164785 ; RRID:Addgene_164785)

  • For your References section:

    A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase. Toth AD, Garger D, Prokop S, Soltesz-Katona E, Varnai P, Balla A, Turu G, Hunyady L. J Biol Chem. 2021 Feb 2:100366. doi: 10.1016/j.jbc.2021.100366. 10.1016/j.jbc.2021.100366 PubMed 33545176
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