A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase.
Toth AD, Garger D, Prokop S, Soltesz-Katona E, Varnai P, Balla A, Turu G, Hunyady L
J Biol Chem. 2021 Feb 2:100366. doi: 10.1016/j.jbc.2021.100366. PubMed Article
Toth AD, Garger D, Prokop S, Soltesz-Katona E, Varnai P, Balla A, Turu G, Hunyady L
J Biol Chem. 2021 Feb 2:100366. doi: 10.1016/j.jbc.2021.100366. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164783 | GLuc-PM | Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors |
| 164784 | NanoLuc-PM | Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors |
| 164785 | GLuc-PM-Venus | Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein |
| 164786 | NanoLuc-PM-Venus | Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein |