pCB301TOR-GFP
(Plasmid
#164799)
-
PurposeExpression of GFP in oomycetes using Agrobacterium-mediated transformation
-
Depositing Lab
-
Depositing OrganizationUniversity of Hawaii
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 164799-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 164799-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepCB301
-
Vector typeOomycete expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer gaattcATGGTGAGCAAGGGCGAG
- 3′ sequencing primer actagtTTACTTGTACAGCTCGTCCATGC
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 164799-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 164799-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCB301TOR-GFP was a gift from Miaoying Tian (Addgene plasmid # 164799 ; http://n2t.net/addgene:164799 ; RRID:Addgene_164799) -
For your References section:
Establishment of a simple and efficient Agrobacterium-mediated transformation system for Phytophthora palmivora. Wu D, Navet N, Liu Y, Uchida J, Tian M. BMC Microbiol. 2016 Sep 6;16(1):204. doi: 10.1186/s12866-016-0825-1. 10.1186/s12866-016-0825-1 PubMed 27599726
