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(Plasmid #164943)


Item Catalog # Description Quantity Price (USD)
Plasmid 164943 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4179
  • Total vector size (bp) 11696
  • Vector type
    Tol2 transposon destination vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Mutation
    Stop codon deleted; Q153H; M154I
  • Entrez Gene
    kcnj13 (a.k.a. ja, kir7, kir7.1, ob)
  • Promoter actinb (actb2) promoter
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2-actinb-kcnj13-Q153H-EGFP was a gift from GuangJun Zhang (Addgene plasmid # 164943 ; ; RRID:Addgene_164943)
  • For your References section:

    Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish. Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, Mittal SK, Zhang G. Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16. 10.1534/genetics.120.303390 PubMed 32546498