p5E-pax3a-5.4k
(Plasmid
#164966)
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Purposezebrafish pax3a -5.4k promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep5E
- Backbone size w/o insert (bp) 2784
- Total vector size (bp) 8196
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Vector typeGateway 5 end entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepax3a
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)5412
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Mutationpromoter -5.4k
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Entrez Genepax3a (a.k.a. pa, pax3, zgc:92547)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E-pax3a-5.4k was a gift from GuangJun Zhang (Addgene plasmid # 164966 ; http://n2t.net/addgene:164966 ; RRID:Addgene_164966) -
For your References section:
Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish. Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, Mittal SK, Zhang G. Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16. 10.1534/genetics.120.303390 PubMed 32546498
