pGEMHE-POLArISact
(Plasmid
#164970)
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PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequence
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEMHE
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Vector typein vitro Transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePOLArISact
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Alt namecpGFP-Adhiron (connected with an alpha helical linker)
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SpeciesSynthetic
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Insert Size (bp)1014
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.06.20.116178v2 full for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEMHE-POLArISact was a gift from Sumio Terada (Addgene plasmid # 164970 ; http://n2t.net/addgene:164970 ; RRID:Addgene_164970) -
For your References section:
POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos. Sugizaki A, Sato K, Chiba K, Saito K, Kawagishi M, Tomabechi Y, Mehta SB, Ishii H, Sakai N, Shirouzu M, Tani T, Terada S. Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). pii: 2019071118. doi: 10.1073/pnas.2019071118. 10.1073/pnas.2019071118 PubMed 33674463
