POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos.
Sugizaki A, Sato K, Chiba K, Saito K, Kawagishi M, Tomabechi Y, Mehta SB, Ishii H, Sakai N, Shirouzu M, Tani T, Terada S
Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). pii: 2019071118. doi: 10.1073/pnas.2019071118. PubMed Article
Sugizaki A, Sato K, Chiba K, Saito K, Kawagishi M, Tomabechi Y, Mehta SB, Ishii H, Sakai N, Shirouzu M, Tani T, Terada S
Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). pii: 2019071118. doi: 10.1073/pnas.2019071118. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164970 | pGEMHE-POLArISact | T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence |
| 164971 | POLArISact | Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells |
| 165044 | cpGFP-Adhiron_flex | Flexible linker control for POLArISact expression in mammalian cells |