pLJM1 FLCN (F118D) FLAG GFP
(Plasmid #164979)

• Purpose: Lentiviral vector for FLCN expression in mammalian cells
• Depositing Lab: Roberto Zoncu
• Publication: Lawrence et al Science. 2019 Nov 22;366(6468):971-977. doi: 10.1126/science.aax0364. Epub 2019 Oct 31.

Backbone

• Vector backbone: PLJM1
• Modifications to backbone: CMV promoter was switched to UBC promoter
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLCN
• Alt name: Folliculin
• Species: H. sapiens (human)
• Mutation: F118D
• Entrez Gene: FLCN (a.k.a. BHD, DENND8B, FLCL)
• Promoter: UBC
• Tag / Fusion Protein:
  • 1xFLAG-GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: hUBCpro-F: TGAAGCTCCGGTTTTGAACT
• 3′ sequencing primer: pLJM1-R: GACGTGAAGAATGTGCGAGA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry