pCAG-TWIN-STREP-FLAG-NPRL3
(Plasmid #164984)

• Purpose: NPRL3 expressing vector for mammalian cells
• Depositing Lab: Roberto Zoncu
• Publication: Lawrence et al Science. 2019 Nov 22;366(6468):971-977. doi: 10.1126/science.aax0364. Epub 2019 Oct 31.

Backbone

• Vector backbone: pCAG TWIN STREP FLAG
• Backbone size w/o insert (bp): 4960
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NPRL3
• Species: H. sapiens (human)
• Insert Size (bp): 1710
• Entrez Gene: NPRL3 (a.k.a. C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11)
• Promoter: CAG
• Tag / Fusion Protein:
  • 2xSTREP FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pCAG-F: CTTCTTCTTTTTCCTACAGCTCCTGGGC
• 3′ sequencing primer: pCAG-R: CAGTGGTATTTGTGAGCCAGGGCATT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry