pLJM1-Flag-Rheb
(Plasmid #165031)

• Purpose: expression of Flag tagged Rheb protein in mammalian cells
• Depositing Lab: Ken Inoki
• Publication: Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. doi: 10.1016/j.molcel.2020.10.004.

Backbone

• Vector backbone: pLJM1
• Backbone manufacturer: Available at Addgene (#19319)
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 7955
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rheb
• Species: H. sapiens (human)
• Insert Size (bp): 555
• GenBank ID: NM_005614.4
• Entrez Gene: RHEB (a.k.a. RHEB2)
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry