pET32a-Spike-S-4P
(Plasmid #165094)

Purpose

protein expression in E. coli
Depositing Lab

Rick Tarleton
Publication

Shen et al medRxiv 2021.(pre-print) 01.20.21249279 ( How to cite )
Sequence Information
- Sequences (3)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	165094	Standard format: Plasmid sent in bacteria as agar stab	1	$75 *	Add to Cart

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Backbone

Vector backbone
pET-32a(+)
(Search Vector Database)
Vector type
Bacterial Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166
Species
SARS-CoV2
Entrez Gene
S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

Cloning method Ligation Independent Cloning

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Industry MTA

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pET32a-Spike-S-4P was a gift from Rick Tarleton (Addgene plasmid # 165094 ; http://n2t.net/addgene:165094 ; RRID:Addgene_165094)
For your References section:

A flexible, pan-species, multi-antigen platform for the detection and monitoring of SARS-CoV-2-specific antibody responses. Shen H, Forgacs D, Chapla D, Moremen KW, Wells L, Hamer SA, Tompkins SM, Ross TM, Rouphael N, Edupuganti S, Collins MH, Tarleton RL.. medRxiv 2021.(pre-print) 01.20.21249279 10.1101/2021.01.20.21249279

pET32a-Spike-S-4P (Plasmid #165094)