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pGoldenGreenGate-M (pGGG-M)
(Plasmid #165422)


Item Catalog # Description Quantity Price (USD)
Plasmid 165422 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pGoldenGreenGate (pGGG) (pGreenII based level 2 Golden Gate (MoClo) compatible vector with additional LB)
  • Backbone size (bp) 2678
  • Modifications to backbone
    Additional LB sequence.
  • Vector type
    Synthetic Biology ; Level 2 Golden Gate (MoClo) dual/binary vector used for Agrobacterium transformation of plants
  • Promoter Contain GG cassette and MCS with LacZ for blue/white screening
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
  • 3′ sequencing primer RB-R1 Reverse 5’-CCCGCCAATATATCCTGTCA-3’
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: This plasmid is based on pGreen and therefore requires the helper plasmid pSoup (Addgene ID # 165419) to replicate in Agrobacterium.

Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGoldenGreenGate-M (pGGG-M) was a gift from John Innes Centre - Crop Transformation and Genome Editing Group & Wendy Harwood (Addgene plasmid # 165422 ; ; RRID:Addgene_165422)
  • For your References section:

    CRISPR-Cas9 Based Genome Editing in Wheat. Smedley MA, Hayta S, Clarke M, Harwood WA. Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65. 10.1002/cpz1.65 PubMed 33687760