Level 1 P2 OsUbiP:Cas9:NosT
(Plasmid #165424)

• Purpose: GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette
• Depositing Labs: John Innes Centre - Crop Transformation and Genome Editing Group, Wendy Harwood
• Publication: Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.1002/cpz1.65.

Backbone

• Vector backbone: pICH47742
• Backbone manufacturer: Icon Genetics
• Backbone size w/o insert (bp): 4352
• Total vector size (bp): 10784
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Os Ubiquitin promoter :: Cas9 :: NosTerminator
• Alt name: RUbiP::Cas9::NosT
• Species: Synthetic
• Insert Size (bp): 6432
• Promoter: Os Ubiquitin promoter :: Cas9 :: NosTerminator

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Golden Gate BsaI (destroyed during cloning)
• 3′ cloning site: Golden Gate BsaI (destroyed during cloning)
• 5′ sequencing primer: LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
• 3′ sequencing primer: RB Reverse 5'-CTGGTGGCAGGATATATTGTGGTG-3'

Resource Information

• Supplemental Documents:
  • level 1 P2-OsUbiP Cas9 NosT.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry