CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
(Plasmid #165436)

• Purpose: Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.
• Depositing Lab: Hideji Murakoshi
• Publication: Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 10.1038/s41467-021-21025-6.

Backbone

• Vector backbone: Customized Vector
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2271
• Mutation: H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residues 60-113 with H83L, H86L, and Q108L mutations) with these mutations does not bind to Cdc42
• Promoter: CMV
• Tag / Fusion Protein:
  • ShadowY, Clover(T153M/F223R) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry