Addgene: Photoactivatable CaMKII induces synaptic plasticity in single synapses.

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Hideji Murakoshi
Shibata et al

Photoactivatable CaMKII induces synaptic plasticity in single synapses.
Shibata ACE, Ueda HH, Eto K, Onda M, Sato A, Ohba T, Nabekura J, Murakoshi H
Nat Commun. 2021 Feb 2;12(1):751. doi: 10.1038/s41467-021-21025-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
165429	pAAV-CaMP0.4-FHS-paCaMKII-WPRE3	Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.
165430	pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3	Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.
165431	CMV-tdTomato-P2A-paCaMKII	Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.
165432	CMV-tdTomato-P2A-paCaMKII(T286A)	Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.
165433	CMV-tdTomato-P2A-paCaMKII(K42M)	Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.
165434	CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42	Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.
165435	CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA	Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.
165436	CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42	Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.
165437	CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA	Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.
165438	CMV-mEGFP(A206K)-paCaMKII	Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.
165439	CMV-mEGFP(A206K)-paCaMKII(T286A)	Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.
165440	CMV-mEGFP(A206K)-paCaMKII(K42M)	Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.
167475	CMV-mEGFP(A206K)-paCaMKII-ShadowG	Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.
167476	CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG	Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.
167477	CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG	Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.
167478	CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG	Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.
167479	CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG	Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Antibodies from Article