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TANGO1S-mScarlet-i
(Plasmid #165461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165461 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 165461-D50 50 μg of DNA in Tris buffer $495
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DNA 165461-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIRES_neo3
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6541
  • Modifications to backbone
    IRES removed
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TANGO1S
  • Alt name
    Mia3
  • Species
    H. sapiens (human)
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet-i (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV_AfeI (unknown if destroyed)
  • 3′ cloning site AgeI_BstXI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TANGO1S cDNA was kindly provided by Vivek Malhotra (CRG, Barcelona)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TANGO1S-mScarlet-i was a gift from David Stephens (Addgene plasmid # 165461 ; http://n2t.net/addgene:165461 ; RRID:Addgene_165461)

  • For your References section:

    A general role for MIA3/TANGO1 in secretory pathway organization and function. McCaughey J, Stevenson NL, Mantell JM, Neal CR, Paterson A, Heesom K, Stephens DJ. J Cell Sci. 2021 Aug 5. pii: 271201. doi: 10.1242/jcs.259075. 10.1242/jcs.259075 PubMed 34350936
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