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(Plasmid #165465)


Item Catalog # Description Quantity Price (USD)
Plasmid 165465 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pCMV-3Tag-8 modified MCS
  • Backbone manufacturer
    Stratagene /Agilent
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    DExD-box helicase 50
  • Alt name
    DDX50, GU2, GUB, mcdrh, RH-II/GuB
  • Alt name
    BC000272.1, NM_024045.1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    DDX50 (a.k.a. GU2, GUB, MGC3199, RH-II/GuB)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV, (T3)
  • 3′ sequencing primer T7, BGH polyA reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Harvard Institute of Proteomics clone HsCD00419808, The ORFeome Collaboration, Broad Clone ID ccsbBroad304_04024, (cDNA clone MGC:3199 IMAGE:3357684).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone contains new multiple cloning site and c-terminal HA epitope replaces FLAGs. Construction: Xhoi-gcc-[DDX50 CDS 2211 bp, no stop]-Noti-a-Clai-HA tag-ctt-STOP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV3Tag8li-hDDX50-HA was a gift from Martin Dorf (Addgene plasmid # 165465 ; ; RRID:Addgene_165465)
  • For your References section:

    TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28. 10.1073/pnas.1322545111 PubMed 24778252