pCMV3Tag8li-hDDX50-HA
(Plasmid #165465)

• Purpose: Human DDX50 mammalian expression vector with C-terminal HA tag.
• Depositing Lab: Martin Dorf
• Publication: Li et al Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28.

Backbone

• Vector backbone: pCMV-3Tag-8 modified MCS
• Backbone manufacturer: Stratagene /Agilent
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DExD-box helicase 50
• Alt name: DDX50, GU2, GUB, mcdrh, RH-II/GuB
• Alt name: BC000272.1, NM_024045.1
• Species: H. sapiens (human)
• Insert Size (bp): 2211
• Entrez Gene: DDX50 (a.k.a. GU2, GUB, MGC3199, RH-II/GuB)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV, (T3)
• 3′ sequencing primer: T7, BGH polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Harvard Institute of Proteomics clone HsCD00419808, The ORFeome Collaboration, Broad Clone ID ccsbBroad304_04024, (cDNA clone MGC:3199 IMAGE:3357684).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone contains new multiple cloning site and c-terminal HA epitope replaces FLAGs. Construction: Xhoi-gcc-[DDX50 CDS 2211 bp, no stop]-Noti-a-Clai-HA tag-ctt-STOP