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(Plasmid #44975)


Item Catalog # Description Quantity Price (USD)
Plasmid 44975 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    DDX3X (a.k.a. CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original plasmid containing the DDX3 gene was obtained from Arvind Patel. It was published in Owsianka and Patel Virology 1999: 257, 330.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-DDX3 was a gift from Robin Reed (Addgene plasmid # 44975 ; ; RRID:Addgene_44975)
  • For your References section:

    Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Lee CS, Dias AP, Jedrychowski M, Patel AH, Hsu JL, Reed R. Nucleic Acids Res. 2008 Aug;36(14):4708-18. doi: 10.1093/nar/gkn454. Epub 2008 Jul 15. 10.1093/nar/gkn454 PubMed 18628297