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dCas9-dMSK1
(Plasmid #165602)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 165602 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LentiCRISPR v2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3 is also a suitable growth strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802)
  • Species
    H. sapiens (human), Synthetic; S. Pyogenes
  • Insert Size (bp)
    6441
  • GenBank ID
    NM_001322227.2
  • Entrez Gene
    RPS6KA5 (a.k.a. MSK1, MSPK1, RLPK)
  • Promoter EF1a
  • Tag / Fusion Protein
    • FLAG Tag (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas9-dMSK1 was a gift from Isaac Hilton (Addgene plasmid # 165602 ; http://n2t.net/addgene:165602 ; RRID:Addgene_165602)
  • For your References section:

    Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Li J, Mahata B, Escobar M, Goell J, Wang K, Khemka P, Hilton IB. Nat Commun 12, 896 (2021). 10.1038/s41467-021-21188-2