PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||165916||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2967
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameSelectable LexAOp Response Vector
Insert Size (bp)7146
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Terminal Alpha Entry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBlastR-12xLexAOp-sfGFP-MiniWhite was a gift from Koen Venken (Addgene plasmid # 165916 ; http://n2t.net/addgene:165916 ; RRID:Addgene_165916)
For your References section:Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila. Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT. Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. 10.1016/j.celrep.2021.109700 PubMed 34525356