Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.
Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. PubMed Article
Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165773 | p3xP3-Hsp70b | HSP70B Promoter fused to synthetic 3xP3 eye driver |
| 165774 | pMCS-TCCC | Multiple cloning site part for enhancer cloning, requires minimal promoter element |
| 165775 | 5xGMR | 5xGMR syntethic eye driver enhancer, requires minimal promoter element |
| 165776 | pdSCP | Minimal synthetic Drosophila core promoter, requires enhancer element for full expression |
| 165777 | pDmActin5c | Drosophila Actin 5C promoter |
| 165779 | p3xP3 | 3xP3 eye driver enhancer element, requires promoter element for function |
| 165780 | pDmHsp70b-TACT | HSP70B promoter for fusion with bacterial promoters |
| 165781 | pDmAlfaTub84b | Drosophila Alpha Tubulin promoter |
| 165783 | pDmVasa | Drosohphila vasa germline promoter and 5'UTR |
| 165785 | pDmHsp70b | Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock |
| 165786 | pDmPolyUbiq | Drosophila Polyubiquitin promoter, strongest promoter in the library |
| 165787 | Promoterless TP10 | Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors |
| 165790 | pPromoterless-P2A | Viral P2A part in promoter grammar, for building promoterless control vectors |
| 165791 | pR20A02 | GB2.0 compatible FlyLight Enhancer element R20A02 in enhancer grammar |
| 165792 | pR70B04 | GB2.0 compatible FlyLight Enhancer element R70B04 in enhancer grammar |
| 165793 | pR76H03 | GB2.0 compatible FlyLight Enhancer element R76H03in enhancer grammar |
| 165794 | pCP6-Dros | Fusion grammar bacterial CP6 promoter, assembles with pHSP70B-TACT |
| 165795 | pCP6-STD | Standard grammar bacterial CP6 promoter |
| 165796 | pCP7-Dros | Fusion grammar bacterial CP7 promoter, assembles with pHSP70B-TACT |
| 165797 | pTP10 | Drosophila TP10 poly(a) terminator element |
| 165798 | pSV40L | Viral Simian virus 40 Late poly(a) terminator element |
| 165800 | pAdh 3'UTR Short | Drosophila HSP70B 3'UTR sequence |
| 165801 | pHSV-TK | Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene |
| 165803 | prrnBT1_STD | Bacterial rrnB gene T1 terminator |
| 165804 | prrnBT2_STD | Bacterial rrnB gene T2 terminator |
| 165805 | pL3S2P21_STD | Synthetic bacterial terminator |
| 165806 | pspy_STD | bacterial terminator |
| 165807 | pGGGSx4 | Flexible peptide linker of 4 repeats of GGGS, for assembly of TL1 to TL5 parts |
| 165809 | pT2A | Viral T2A linker element for assembly of TL3 to TL5 parts |
| 165810 | pGGSx4 NT | Flexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 parts |
| 165811 | pGGSx4 CT | Flexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 parts |
| 165814 | p4xLexAOp-A1 | Alpha1 4xLexAOp for assembly of larger LexAOp response elements |
| 165815 | p4xLexAOp-A2 | Alpha2 4xLexAOp for assembly of larger LexAOp response elements |
| 165816 | p4xLexAOp-O2 | Omega2 4xLexAOp for assembly of larger LexAOp response elements |
| 165817 | p4xLexAOp-O1 | Omega1 4xLexAOp for assembly of larger LexAOp response elements |
| 165818 | p5xQUAS | Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly |
| 165819 | pGal4BD 1-147 | 1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain |
| 165820 | pGAL4AD 768-881 | 768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain |
| 165821 | pLexA BD | LexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain |
| 165822 | pVP16 AD | VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain |
| 165823 | pQFBD | QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain |
| 165824 | pQFAD | QF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain |
| 165825 | pGAL80 | GAL4 inhibitor coding sequence (CDS) |
| 165826 | pQS | QF inhibitor coding sequence (CDS) |
| 165827 | pSV40L-TN | Viral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRs |
| 165828 | pmCherry | mCherry red fluorescent protein CDS |
| 165829 | psfGFP | sfGFP green fluorescent protein CDS |
| 165830 | pEGFP | EGFP green fluorescent protein CDS |
| 165831 | pEBFP2 | EBFP2blue fluorescent protein CDS |
| 165832 | pmCherry TL1 | mCherry red fluorescent protein for N-terminal tagging |
| 165833 | pmCherry TL5 | mCherry red fluorescent protein for C-terminal tagging |
| 165834 | pEGFP TL1 | sfGFP green fluorescent protein for N-terminal tagging |
| 165835 | pEGFP TL5 | sfGFP green fluorescent protein for C-terminal tagging |
| 165836 | pNPTII | G418 sulfate (geneticin) resistance marker |
| 165837 | pPAC | puromycin resistance marker |
| 165838 | pBSR | blasticidin S resistance marker |
| 165839 | pHPH | hygromycin B resistance marker |
| 165840 | pBLE | Zeocin/Phleomycin resistance marker |
| 165841 | pTK | ganciclovir sensitivity marker |
| 165842 | pFCU1 | 5-fluorocytosine sensitivity marker |
| 165843 | pFC31 attB O1 | _C31 integrase attB site in pColE1_Omega1 |
| 165844 | pFC31 attB O2 | _C31 integrase attB site in pColE1_Omega2 |
| 165845 | pFC31 | _C31 integrase CDS |
| 165846 | pWhite_CDS | White gene CDS |
| 165856 | pUPD2 | Universal Part domesticator vector v2 used for integration (domestication) of new DNA elements in the GB2.0 cloning system uses blue-white screening |
| 165857 | pVD2 | universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method |
| 165858 | P[acman]-Alpha1 | Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening |
| 165859 | P[acman]-Alpha2 | Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening |
| 165860 | P[acman]-Omega1 | Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening |
| 165861 | P[acman]-Omega2 | Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening |
| 165862 | pR6Kg-Alpha1 | Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening |
| 165863 | pR6Kg-Alpha2 | Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening |
| 165864 | pR6Kg-Omega1 | Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) |
| 165865 | pR6Kg-Omega2 | Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening |
| 165866 | pR6Kg-Alpha1spm | Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening |
| 165867 | pColE1-AlphaOmega | Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening |
| 165868 | pEGFP-NT-Tagging | Template for amplification of EGFP N-terminal tagging cassette |
| 165869 | pmCherry-CT-Tagging | Template for amplification of mcherry C-terminal tagging cassette |
| 165870 | pmCherry-NT-Tagging | Template for amplification of mCherry N-terminal tagging cassette |
| 165871 | pEGFP-CT-Tagging | Template for amplification of EGFP C-terminal tagging cassette |
| 165872 | pMiniwhiteA1 | Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site |
| 165873 | pMiniWhiteA2 | Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site |
| 165874 | pGMR-WhiteA2 | Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site |
| 165875 | p3XP3-WhiteA2 | Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site |
| 165876 | pHSP70B-CP6-nptII | Basic G418 resistance cassette in Alpha1, lacks physical marker or attB site |
| 165877 | pHSP70B-CP6-pac | Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site |
| 165878 | pHSP70B-CP6-bsr | Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site |
| 165879 | pHSP70B-CP6-hph | Basic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB site |
| 165880 | pHSP70B-CP6-ble | Basic Phleomycin resistance cassette in Alpha1, lacks physical marker or attB site |
| 165881 | pHSP70B-CP6-TK | Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site |
| 165882 | pHSP70B-CP6-FCU1 | Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site |
| 165883 | pG418R-GMRWhite | G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening |
| 165884 | pPuroR-GMRWhite | Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening |
| 165885 | pBlastR-GMRWhite | Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening |
| 165886 | pHygroR-GMRWhite | Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening |
| 165887 | pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31 | G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions |
| 165888 | pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31 | Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions |
| 165889 | pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31 | Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions |
| 165890 | pPuroR-MCS-GAL4-MiniWhite | Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening. |
| 165891 | p5xUAS | Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly |
| 165894 | pG418R-MCS-LexA-VP16-MiniWhite | G418 resistant LexA driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening. |
| 165898 | pPuroR-MCS-LexA-VP16-MiniWhite | Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning |
| 165900 | pBlastR-MCS-LexA-VP16-MiniWhite | Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning |
| 165902 | pG418R-MCS-QF-MiniWhite | G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning |
| 165906 | pBlastR-MCS-QF-MiniWhite | Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning |
| 165907 | pBlastR-5xUAS-sfGFP-GMRWhite | Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165908 | pBlastR-5xUAS-mCherry-GMRWhite | Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165910 | pBlastR-5xUAS-sfGFP-MiniWhite | Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165911 | pBlastR-5xUAS-mCherry-MiniWhite | Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165912 | pBlastR-5xUAS-EBFP2-MiniWhite | Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165916 | pBlastR-12xLexAOp-sfGFP-MiniWhite | Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165917 | pBlastR-12xLexAOp-mCherry-MiniWhite | Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165918 | pBlastR-12xLexAOp-EBFP2-MiniWhite | Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165920 | pBlastR-5xQUAS-mCherry-GMRWhite | Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165921 | pBlastR-5xQUAS-EBFP2-GMRWhite | Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165922 | pBlastR-5xQUAS-sfGFP-MiniWhite | Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165923 | pBlastR-5xQUAS-mCherry-MiniWhite | Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |
| 165924 | pBlastR-5xQUAS-EBFP2-MiniWhite | Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening |