Purpose(Empty Backbone) Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||165862||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 1941
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pR6Kg-Alpha1 was a gift from Koen Venken (Addgene plasmid # 165862 ; http://n2t.net/addgene:165862 ; RRID:Addgene_165862)
For your References section:Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila. Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT. Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. 10.1016/j.celrep.2021.109700 PubMed 34525356