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pLENTICRISPR-HK2-sgRNA2
(Plasmid #165930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165930 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLENTICRISPR v2
  • Backbone manufacturer
    Feng Zhang, Addgene 52966
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HK2
  • gRNA/shRNA sequence
    CACCGTTACTTTCACCCAAAGCACA
  • Species
    H. sapiens (human)
  • Entrez Gene
    HK2 (a.k.a. HKII, HXK2)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLENTICRISPR-HK2-sgRNA2 was a gift from Vamsi Mootha (Addgene plasmid # 165930 ; http://n2t.net/addgene:165930 ; RRID:Addgene_165930)

  • For your References section:

    Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from glycolysis to OXPHOS. Alexis A. Jourdain, Bridget E. Begg, Eran Mick, Hardik Shah, Sarah E. Calvo, Owen S. Skinner, Rohit Sharma, Steven M. Blue, Gene W. Yeo, Christopher B. Burge, Vamsi K. Mootha 8. Molecular Cell 10.1016/j.molcel.2021.02.033
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