pET24_NusA-Tus
(Plasmid #165959)

• Purpose: Expression of soluble E. coli Tus protein
• Depositing Lab: Keith Pardee
• Publication: Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.1021/acssynbio.1c00110.

Backbone

• Vector backbone: pET24b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5240
• Total vector size (bp): 7715
• Modifications to backbone: Originally modified from pET24b-APEX2 Addgene #111702
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tus
• Alt name: DNA replication terminus site-binding protein
• Species: Escherichia coli
• Insert Size (bp): 2475
• GenBank ID: BAA15348.1
• Promoter: T7
• Tag / Fusion Protein:
  • His-NusA-Tev (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 FW
• 3′ sequencing primer: T7 RV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid backbone is originally pET24b-APEX2 (Plasmid #111702) acquired from Addgene. In this submission, the Apex2 insert has been replaced with NusA-Tus.