pGK401red-rtetTAK8N, L131L
(Plasmid
#165968)
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PurposeExpresses rTetTA (K8N, L131L) mutant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGK401red
- Total vector size (bp) 6713
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersMET15
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametetR
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SpeciesSynthetic; Escherichia coli
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Tag
/ Fusion Protein
- three copies of VP16 (VP48) (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTet systems (pTet-On Advanced).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The synthetic transcription activator (rtTA) is originated from Tet systems (pTet-On Advanced, Tet systems).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGK401red-rtetTAK8N, L131L was a gift from Jun Ishii (Addgene plasmid # 165968 ; http://n2t.net/addgene:165968 ; RRID:Addgene_165968) -
For your References section:
Robust and flexible platform for directed evolution of yeast genetic switches. Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A. Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y. 10.1038/s41467-021-22134-y PubMed 33758180
