|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||166089||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSmith JD et al 2016. PMID 26956608
Vector typeYeast Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Entrez GeneGAL7 (a.k.a. YBR018C)
- Promoter Tet-inducible
- Cloning method Gibson Cloning
gRNA Binding Coordinates: chrII:275406..275425 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNSU299_GAL7 was a gift from Rebecca Burgess (Addgene plasmid # 166089 ; http://n2t.net/addgene:166089 ; RRID:Addgene_166089)