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Rebecca Burgess Lab: Burgess Lab Plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
166071pNSU299_CPR1_2Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
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166072pNSU299_YDR344CPlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
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166073pNSU299_APL3Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast
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166074pNSU299_CPR1_1Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
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166075pNSU299_SLA1Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast
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166076pNSU299_SEO1Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
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166077pNSU299_YBL055CPlasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast
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166078pNSU299_RPL39Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
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166079pNSU299_RPL22APlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
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166080pNSU299_RRT8Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
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166081pNSU299_YNR071CPlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.
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166082pNSU299_TDH2Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast
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166083pNSU299_PPE1Plasmid for constituive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast
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166084pNSU299_TDH3Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.
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166085pNSU299_LYS9Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast
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166086pNSU299_CUP1-1Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
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166088pNSU299_GAL2Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
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166089pNSU299_GAL7Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
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166090pNSU299_GLK1Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
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166091pNSU299_MET16Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
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166092pNSU299_LHS1Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
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166093pNSU299_PDR12Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast
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166094pITI1Gal inducible expression of dCas9-FokI
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166095pITI2Gal inducible expression of D10A-Cas9 nickase
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166096pRR1Gal inducible expression of Cas9 WT
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166097pRR2Gal inducible expression of dCas9-FokI
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166098pPH3Gal inducible expression of D10A-Cas9 nickase
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166099pMEL15_MATzExpression of gRNA targeting the MAT locus
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166100pROS15_MATzExpression of dual gRNA targeting the MAT locus
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