pITI2
(Plasmid #166095)

• Purpose: Gal inducible expression of D10A-Cas9 nickase
• Depositing Lab: Rebecca Burgess
• Publication: Burgess Lab Plasmids (unpublished)

Backbone

• Vector backbone: p415
• Backbone manufacturer: Addgene 43804
• Vector type: Yeast Expression, CRISPR
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9-D10A
• Species: Synthetic; S pyogenes Cas9
• Mutation: spCas9-D10A
• Promoter: Gal-inducible

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • piti2.gb
• A portion of this plasmid was derived from a plasmid made by: 273 bp of Cas9 containing D10A amplified from Addgene plasmid 64279 and cloned into Addgene plasmid 43804

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry