pEGFP_WT-talin1 (1-2541)
(Plasmid
#166112)
-
PurposeFor expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.
-
Depositing Lab
-
Depositing OrganizationTampere University, BioMediTech
Located in Finland -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 166112-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 166112-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepEGFP-C1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4734
- Total vector size (bp) 12357
-
Vector typeMammalian Expression ; CMV promoter
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsFor MidiPrep according to the instructions from the kit.
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameWT-Talin-1
-
Alt nameTLN1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)7623
-
Entrez GeneTln1 (a.k.a. Tln)
-
Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 166112-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 166112-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP_WT-talin1 (1-2541) was a gift from Vesa Hytönen (Addgene plasmid # 166112 ; http://n2t.net/addgene:166112 ; RRID:Addgene_166112) -
For your References section:
Cancer associated talin point mutations disorganise cell adhesion and migration. Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytonen VP. Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4. 10.1038/s41598-020-77911-4 PubMed 33431906
Map uploaded by the depositor.
Map uploaded by the depositor.
