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pEGFP_∆DD-talin1 (1-2493)
(Plasmid #166124)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4734
  • Total vector size (bp) 12357
  • Vector type
    Mammalian Expression ; CMV promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For MidiPrep according to the instructions from the kit.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ∆DD-Talin-1
  • Alt name
    ∆DD-TLN1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7623
  • Mutation
    residues 1-2493, deletion of dimerization domain (∆DD)
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP_∆DD-talin1 (1-2493) was a gift from Vesa Hytönen (Addgene plasmid # 166124 ; http://n2t.net/addgene:166124 ; RRID:Addgene_166124)

  • For your References section:

    Cancer associated talin point mutations disorganise cell adhesion and migration. Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytonen VP. Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4. 10.1038/s41598-020-77911-4 PubMed 33431906
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