DNMT1 KO Neo
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16649||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3264
Vector typeBacterial Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameDNMT1 knock-out construct
SpeciesH. sapiens (human)
Entrez GeneDNMT1 (a.k.a. ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
5' arm is 4.3 kb. 3' arm is 3.0 kb. See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DNMT1 KO Neo was a gift from Bert Vogelstein (Addgene plasmid # 16649 ; http://n2t.net/addgene:16649 ; RRID:Addgene_16649)
For your References section:DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B. Nature. 2002 Apr 4. 416(6880):552-6. 10.1038/416552a PubMed 11932749
Map uploaded by the depositor.