mCerulean3-Bcl-W-FUGW
(Plasmid #166751)

• Purpose: Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.
• Depositing Lab: David Andrews
• Publication: Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. Epub 2022 Apr 20.

Backbone

• Vector backbone: FUGW
• Vector type: Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl-W
• Alt name: Bcl2 like protein 2 (Bcl2l2)
• Species: M. musculus (mouse)
• Entrez Gene: Bcl2l2 (a.k.a. AW048834, Bcl-, Bcl-w, Gtrg, Gtrgal2, Gtro, Gtrosa41, bcl, bclw, c98)
• Promoter: human ubiquitin C
• Tag / Fusion Protein:
  • mCerulean3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • mCerulean3-Bcl-W-FUGW
• A portion of this plasmid was derived from a plasmid made by: mCerulean3 received from Mark Rizzo PMID: 21479270 and cloned into this plasmid. Bcl-W gene sequence aligns with Mus musculus BCL2-like 2 (Bcl2l2), transcript variant X2, mRNA (NCBI Reference Sequence: XM_006518457.5). FUGW backbone from Addgene #14883, where EGFP was replaced by mCerulean3-Bcl-W.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W.