pFastBac HTB DPP9L
(Plasmid #166788)

• Purpose: Insect cell expression vector for DPP9L (protein purification)
• Depositing Lab: Hao Wu
• Publication: Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4.

Backbone

• Vector backbone: pFastBac HTB
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DPP9
• Alt name: DPP9L
• Species: H. sapiens (human)
• Entrez Gene: DPP9 (a.k.a. DP9, DPLP9, DPP IX, DPRP-2, DPRP2, HATIS, IMD111)
• Promoter: polyhedrin
• Tag / Fusion Protein:
  • HIS-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: polyhedrin-fwd
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insect cell expression vector for DPP9L (protein purification)