pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized
(Plasmid #166834)

• Purpose: Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells
• Depositing Lab: Hao Wu
• Publication: Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4.

Backbone

• Vector backbone: pcDNA3.1 LIC 6LD
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLRP1
• Alt name: Codon-optimized NLRP1
• Species: H. sapiens (human)
• Entrez Gene: NLRP1 (a.k.a. AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1)
• Promoter: CMV
• Tag / Fusion Protein:
  • TEV-GFP-FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV F
• 3′ sequencing primer: GFP reverse (5'cagctcgaccaggatgggc3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells