pRS406 ATG1
(Plasmid
#166846)
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PurposeExpresses Atg1 with ATG1 3'UTR in yeasts.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS406
- Total vector size (bp) 9024
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATG1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)4893
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Entrez GeneATG1 (a.k.a. YGL180W, APG1, AUT3, CVT10)
- Promoter ATG1 Promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XmaI (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with StuI
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS406 ATG1 was a gift from Daniel Klionsky (Addgene plasmid # 166846 ; http://n2t.net/addgene:166846 ; RRID:Addgene_166846) -
For your References section:
The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy. Gatica D, Hu G, Liu X, Zhang N, Williamson PR, Klionsky DJ. Mol Cell. 2019 Jan 17;73(2):314-324.e4. doi: 10.1016/j.molcel.2018.11.002. Epub 2018 Dec 6. 10.1016/j.molcel.2018.11.002 PubMed 30527663
